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| Registros recuperados : 14 | |
| 1. |  | MACIEL, D. L.; VARGAS, J. A. C.; MEZZOMO, R.; GAMA, M. A. S. da; LEITE, L. C.; CASTRO, I. R. R. de; OLIVEIRA, L. R. S.; FARIAS, M. L. C.; LUZ, W. B. dos S.; ALVES, K. S. Physicochemical, nutritional, and sensory attributes of 1 Minas frescal cheese from grazing cows fed a supplement containing different levels of babassu coconut (Orbignya speciosa). International Dairy Journal, v. 127, 105176, 2022. Publicado online em: 1 set. 2021.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | CASTRO, I. R. R. de; MACIEL, D. L.; VARGAS, J. A. C.; GOMES, D. I.; MACIEL, R. P.; MEZZOMO, R.; GAMA, M. A. S. da; OLIVEIRA, L. R. S.; GALVÃO, L. T. O.; PEREIRA, K. da S.; ALVES, K. S. Nutrient utilization, performance, and milk fatty acid composition of grazing cows fed supplements with babassu coconut. Tropical Animal Health and Production, v. 53, 433, 2021.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | MEZZOMO, R.; ROLIM, J. M.; SANTOS, A. F. dos; POLETTO, T.; WALKER, C.; MACIEL, C. G.; MUNIZ, M. F. B. Aggressiveness of Fusarium oxysporum and Fusarium solani isolates to yerba-mate and production of extracellular enzymes. Summa Phytopathologica, Botucatu, v. 45, n. 2, p. 141-145, 2019. 5 p.| Biblioteca(s): Embrapa Florestas. |
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| 4. | | MEZZOMO, R.; PAULINO, P. V. R.; BARBOSA, M. M.; MARTINS, T. S.; PEREIRA, L. G. R.; SILVA, J. C.; PAULINO, M. F.; SILVA, M. C.; SERÃO, N. V. Protein dietary efficiency and methane emission in cattle fed soybean meal treated with tannins. Animal Production Science, v. 58, n. 12, p. 2233-2241, 2018.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | MACIEL, C. G.; MUNIZ, M. F. B.; MEZZOMO, R.; REINIGER, L. R. S. Lasiodiplodia theobromae associated with seeds of Pinus spp. originated from the northwest of Rio Grande do Sul, Brazil. Scientia Forestalis, Piracicaba, v. 43, n. 107, p. 639-646, set. 2015.| Biblioteca(s): Embrapa Florestas. |
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| 7. | | LAZAROTTO, M.; MUNIZ, M. F. B.; SANTOS, A. F. dos; MEZZOMO, R.; PIVETA, G.; BLUME, E. Qualidade fisiológica e tratamentos de sementes de cedrela fissilis procedentes do sul do Brasil. Revista Árvore, Viçosa, v. 37, n. 2, p. 201-210, 2013.| Biblioteca(s): Embrapa Florestas. |
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| 8. | | VIEIRA, E. S. N.; SANTOS, A. F. dos; REGO, G. M.; MEZZOMO, R; BORA, K. C. Qualidade sanitária de sementes de araucária de sementes tratadas com hipoclorito de sódio. Informativo ABRATES, v. 21, n. 2, p. 130, 2011. Edição dos resumos do 17º Congresso Brasileiro de Sementes, 2011, Natal. Resumo 1109.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | RIBEIRO, M. E. R.; BARBOSA, R. S.; SEDREZ, F. da S.; MEZZOMO, R.; VON-AHN, C. M. E.; SCHRAMM, R. Influência da CCS e CBT com componentes físico-químicos do leite. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 4., 2010, Florianópolis. Anais... Florianópolis: CBQL, 2010.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | POLETTO, I.; MUNIZ, M. F. B.; CECONI, D. E.; MEZZOMO, R.; RODRIGUES, J. Influência da inoculação de Fusarium spp. e níveis de sombreamento no crescimento e desenvolvimento da erva-mate. Ciência Florestal, Santa Maria, v. 20, n. 3, p. 513-521, jul./set. 2010.| Biblioteca(s): Embrapa Florestas. |
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| 11. | | MEZZOMO, R.; REIS, S. F. dos; PAULINO, P. V. R.; MEDEIROS, S. R. de; CRISTALDO, R. de O.; FEIJO, G. L. D. Rendimentos de cortes comerciais e composição física da carcaça de novilhas de corte de diferentes classes de consumo alimentar residual. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 47., 2010, Salvador. Empreendedorismo e progresso científicos na zootecnia brasileira de vanguarda: anais. Salvador: SBZ, 2010. 1 CD-ROM. 3 p. R4420| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | POLETTO, I.; MUNIZ, M. F. B.; CECONI, D. E.; ARAUJO, M. M.; RODRIGUES, J.; MEZZOMO, R. Inoculação de Fusarium oxysporum e Fusarium solani e níveis de sombreamento na erva-mate: influência na severidade da podridão-de-raízes. Ciência Florestal, Santa Maria, v. 19, n. 3, p. 267-278, jul./set. 2009.| Biblioteca(s): Embrapa Florestas. |
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| 13. | | RIBEIRO, M. E. R.; BARBOSA, R. S.; MEZZOMO, R.; SCHRAMM, R.; VON-AHN, C. M. E.; SEDREZ, F. da S. Avaliação dos parâmetros higiênico-sanitários com influência na qualidade do leite. In: CONGRESSO BRASILEIRO DE NUTRIÇÃO ANIMAL, 1., 2008, Fortaleza. Bioecnologia aplicada na produção de rações: anais. Fortaleza: SEBRAE-CE, 2008. Seção Manejo e Nutrição de Ruminantes. 1 CD-ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | RIBEIRO, M. E. R.; BARBOSA, R. S.; ZANELA, M. B.; GOMES, J. F.; VON-AHN, C. M. E.; SEDREZ, F. da S.; MEZZOMO, R. Efeito do Azidiol e Bronopol em amostras de leite para o diagnóstico do leite instável não ácido (LINA). In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE: SEGURANÇA ALIMENTAR E SAÚDE PÚBLICA, 3., 2008, Recife. Anais... Recife:CBQL: UFRPE, 2008. 1 CD- ROM.| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 14 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/12/2021 |
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Data da última atualização: |
10/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; PEIXOTO, R. M.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; LIMA, A. M. C.; NOBRE, J. A.; AMARAL, G. P.; BRANDÃO, I. S.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO, State University of Ceará - Fortaleza, Ceará, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO, Scholarship for Regional Scientific Development of the National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Level C, Brasilia, Distrito Federal, Brazil; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO, State University of Ceará - Fortaleza, Ceará, Brazil; ANA MILENA CESAR LIMA, Federal University of Piauí - Teresina, Piauí, Brazil; JULIANA ARAÚJO NOBRE, State University of Ceará - Fortaleza, Ceará, Brazil; GABRIEL PAULA AMARAL, State University of Acaraú Valley, Sobral, Ceará, Brazil; IANE SOUSA BRANDÃO, State University of Acaraú Valley, Sobral, Ceará, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, State University of Ceará - Fortaleza, Ceará, Brazil. |
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Título: |
Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 78, 101693, Oct. 2021. |
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DOI: |
https://doi.org/10.1016/j.cimid.2021.101693 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission. MenosAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally i... Mostrar Tudo |
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Palavras-Chave: |
Amniotic sac; Intrauterine transmission; Retrovirus; SRLVs; Vertical disease transmission. |
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Thesaurus NAL: |
Body fluids; Disease diagnosis; Goat diseases; Goats; Risk factors. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02839naa a2200373 a 4500
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cimid.2021.101693$2DOI
100 1 $aARAÚJO, J. F.
245 $aDetection and isolation of small ruminant lentivirus in the amniotic fluid of goats.$h[electronic resource]
260 $c2021
520 $aAbstract:The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ?L was taken from the sediment and another 600 ?L sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.
650 $aBody fluids
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aRisk factors
653 $aAmniotic sac
653 $aIntrauterine transmission
653 $aRetrovirus
653 $aSRLVs
653 $aVertical disease transmission
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aPEIXOTO, R. M.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aLIMA, A. M. C.
700 1 $aNOBRE, J. A.
700 1 $aAMARAL, G. P.
700 1 $aBRANDÃO, I. S.
700 1 $aTEIXEIRA, M. F. da S.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 78, 101693, Oct. 2021.
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